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Journal: bioRxiv
Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection
doi: 10.64898/2026.03.18.712682
Figure Lengend Snippet: (A-B) Graphs show XCR1 (A) and SIRPa (B) signal density within innate-infiltrated and non-infiltrated regions on stained spleen sections harvested 24hrs after LM-OVA infection (n=5). C- Mice were adoptively transferred with OTIxGFP T-cells and infected with LM-OVA. After 24hrs, spleen sections were stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green). Representative image. (D-F) Mice were infected with LM-OVA for 24hrs and splenocytes were analysed by flow cytometry. Graphs show CD80 MFI ( D , n=5-7), CD86 MFI ( E , n=5-7), and frequency of CD70+ cDC1 and cDC2 ( F , n=4-5). (G-H) Spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), CD70 (red), CD11b (white). G- Representative image. H- Graph shows CD70 signal intensity within innate-infiltrated and non-infiltrated regions in WPs (n=5). I-K Mice were infected with LM-OVA and treated with anti-CXCR3 or anti-CD70 antibodies as indicated 6hrs before infection. Graphs show frequencies (I) , absolute numbers (J) and avidity (K) of N4-tetramer+ CD8 T-cells 8 days post-infection (n=12-17 per group). Ratio paired t-tests (A-B, D-F, H) , Welch and Brown-Forsythe one-way ANOVA with Dunnett’s multiple comparisons test (I-K) .
Article Snippet: For CD70 blockade, mice were intraperitoneally administered 300μg
Techniques: Staining, Infection, Flow Cytometry
Journal: bioRxiv
Article Title: Transient immune landscape remodelling shapes CD8 T-cell priming during infection
doi: 10.64898/2026.03.18.712682
Figure Lengend Snippet: (A-B) Representative image of spleen sections from mice infected with LM-OVA for 24hrs were stained for CD169 (blue), XCR1 (cyan), NKp46 (red) and SIRPa ( A , magenta), or 33D1 ( B , magenta). C- Mice were adoptively transferred with OTIxGFP cells and infected with LM-OVA. Representative image of spleen sections stained for CD169 (red), 33D1 (magenta), XCR1 (cyan), OTI (green) 24hrs post-infection. (D-E) Splenocytes from mice infected with LM-OVA for 24hrs were analysed by flow cytometry. D- Representative gating strategy for splenic cDC1 and cDC2 subsets. E- Representative histograms showing CD80, CD86, and CD70 expression in cDC1 and cDC2.
Article Snippet: For CD70 blockade, mice were intraperitoneally administered 300μg
Techniques: Infection, Staining, Flow Cytometry, Expressing